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1.Add 180 μl of Zymo DNA binding buffer to a nuclease-free 1.5 ml centrifuge tube. 2.Set up a Zymo spin column with a collection tube. 3.Transfer 50 μl tagmentation reaction to the 1.5 ml centrifuge tube.
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22/12/2004 · Hello. Problem situation is a Myson Apollo (30Si). It has been in use for probably about ten years. Although working fine, it has started to make a lot of noise.
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The last step of the procedure appears to be a Dieckmann cyclization (intramolecular Claisen condensation) followed by decarboxylation. Additionally, benzilic acid is made in a few steps starting from benzaldehyde and cyanide catalyst.
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